Table 1 Antibodies used in this study

Primary Antibodies

Rabbit polyclonal anti-CD31 (1:200)

Abcam

Cat #: ab28364, RRID:AB_726362

Rat monoclonal anti-CD13, cloneR3-63 (1:250)

Bio-Rad

Cat #: MCA2183T RRID:AB_1100679

Mouse monoclonal anti-CNPase, 11-5B, (1:500)

Sigma-Aldrich

Cat #: C5922, RRID:AB_476854

Mouse monoclonal anti-myelin basic protein (MBP), clone SMI 99, (1:500)

Biolegend

Cat #: 808,402, RRID:AB_2564742

Rabbit polyclonal anti-Collagen-IV biotinylated (1:500)

Abcam

Cat #: ab6581, RRID:AB_305579

Rat monoclonal anti-Transferrin Receptor (1:500)

Novus Biologicals

Cat #: NB100-64979, RRID:AB_962622

Rabbit polyclonal anti-AQP4 (1:5000)

Sigma-Aldrich

Cat #: HPA014784, RRID:AB_1844967

Mouse recombinant anti-GFP [N86/38.1R]

(1:5)

cell-culture supernatant, laboratory

gift from James Trimmer

Addgene plasmid # 114,492,

RRID:Addgene_114492

Secondary Antibodies

Goat polyclonal anti-rabbit Alexa 488 (1:500

Invitrogen

Cat#: A11034, RRID:AB_2576217

Goat polyclonal anti-mouse IgG2A Alexa 488 (1:500)

Invitrogen

Cat#: A21131, RRID:AB_2535771

Goat polyclonal anti-mouse IgG1 Alexa 488 (1:500)

Invitrogen

Cat#: A21121, RRID:AB_2535764

Goat polyclonal anti-rat Alexa 555 (1:500)

Invitrogen

Cat#: A21434, RRID:AB_2535855

Goat polyclonal anti-rabbit Alexa 568 (1:500)

Invitrogen

Cat#: A11036, RRID:AB_10563566

Goat polyclonal anti-mouse IgG1 568 (1:500)

Invitrogen

Cat#: A21124, RRID:AB_2535766

Goat polyclonal anti-rabbit Alexa 647 (1:500)

Invitrogen

Cat#: A21245, RRID:AB_2535813

Goat polyclonal anti-rabbit IgG Atto-647 N (1:500)

Sigma-Aldrich

Cat#: 40,839 RRID:AB_1137669

Goat polyclonal anti-mouse IgG1 Alexa 647 (1:500)

Invitrogen

Cat#: A21240, RRID:AB_2535809

Streptavidins and Lectin

Streptavidin Alexa Fluor 555 (1:500)

Invitrogen

Cat#: S21381, RRID:AB_2307336

Streptavidin Alexa Fluor 594 (1:500)

Jackson Immuno Research

Cat#: 016-580-084 RRID:AB_2337250

Lycopersicon Esculentum (Tomato) Lectin DyLight-649 (1:1000)

Vector Laboratories

Cat #: DL-1178-1