Fig. 5

Remyelination reestablishes vascular association of oligodendrocytes

(A) Schematic of the experimental timeline and schematic overview and locations of the microscopy images shown in B and D (black box). (B) CNPase labeling of the motor cortex reveals that 5 weeks of cuprizone treatment leads to near complete demyelination compared to control. Red boxes indicate the zone of quantification in layer 2/3, a dotted line denotes the cortex surface. (C) Quantifications of the oligodendrocyte density after 5 weeks of demyelination in layer 2/3, compared to age matched controls (12 week old mice, unpaired t-test). (D) Quantifications in layer 2/3 show that vOLs are strongly reduced (unpaired t-test). (E) Example overview images of the cortex from age matched control and remyelinated mice that were labeled with a CNPase antibody. In the remyelinated cortex, myelin still appears less homogeneous compared to control. Red boxes indicate the area of quantification in layer 2/3. (F) Quantifications in layer 2/3 show that after 7 weeks of remyelination, total oligodendrocyte density is still reduced in motor cortex compared to age matched control mice. Age of mice at time of analysis is 22 weeks. Unpaired t-test, p = 0.001. (G) Example of a vOL in motor cortex (arrow head) after 7 weeks of remyelination. (H) Quantifications of vOL density during remyelination show a reduction compared to control. Single data points represent data from one animal. Unpaired t-test, p = 0.024, n = 4 animals for control and for remyelination. (I) Quantifications reveal that the percentage of vOLs during remyelination is comparable to the control motor cortex. Unpaired t-test, p = 0.8, n = 4 animals for control and for remyelination. Single data points in C, D, F, H and I represent averaged data from one animal.