Fig. 6

Mitochondrial movement is reduced in aged choroid plexus epithelial cells. A Schematic of MitoTracker incubation and 2-PM imaging (ACSF: artificial cerebrospinal fluid). B Representative 2-PM images of 3–4 and 22–24 months old (m.o.) epithelial cells. Scale bar = 5 µm. C Manually traced mitochondria in 3–4 and 22–24 m.o. cells. Inserts show a single traced mitochondrion. Quantification of total length traveled by mitochondria (D), mitochondrial displacement at 400 s (E), mitochondrial instantaneous (Inst.) speed (F) and mitochondrial average (Avg) speed at 400 s (G) in 3–4 and 22–24 m.o. n = 50 mitochondria per animal, 4 animals per age; black line: median. E, G: Mann–Whitney; ****p < 0.0001