Fig. 4

Immunofluorescence staining of ABCG2 in methanol/acetone-fixed and methacarn-fixed murine brain. Fresh frozen sections of the dorsal third ventricle were prepared from wild-type (WT) and ABCG2 knockout (KO) mice and post-fixed with methanol and acetone. Immunofluorescence staining of ABCG2 was seen in choroid plexus and capillaries in sections from a WT but not in b ABCG2 KO mouse. Scale bar 100 µm. c Immunofluorescence staining of ABCG2 (magenta) and GLUT9 (green) showing ABCG2 in the choroid plexus and GLUT9 in the ependyma. Scale bar 100 µm. d–f Immunofluorescence staining of d ABCG2 and e GLUT9 using methacarn-fixed paraffin section of capillaries showing co-localization in f. Blue indicates DAPI-stained nucleus of the brain capillary endothelial cell. Scale bar 10 µm. CP, choroid plexus; D3V, dorsal third ventricle