Fig. 4

Essential-8 medium does not impact BMECs differentiation. a IMR90 iPSC growth curve on C-Matrigel (orange), L-Matrigel (red), T-Matrigel (purple) or vitronectin (green)-coated surfaces. IMR90 iPSCs were seeded as single cells at day 0 at a density of 20 × 10³ cells/cm² on the different types of matrices and maintained in E-8 medium. At each timepoint (day), IMR90 iPSC colonies were dissociated as single cells using Accutase and counted with a cell hemocytometer using 0.4 % Trypan blue as an exclusion dye. b Cell yield at day 8 of differentiation. Cell yield was determined by dividing average density at day 8 of differentiation by the initial cell seeding density at day 0. c, d TEER and sodium fluorescein permeability values on purified iPSC-derived BMECs monolayers 48 h after purification. Note the poor barrier properties in cells differentiated on Geltrex (L-Matrigel) or on vitronectin-N (vitronectin), * and ** denote P < 0.05 and P < 0.01 in comparison to C-Matrigel