Fig. 1
Effects of Matrigel origin on IMR90 iPS cell growth and BMEC differentiation using mTeSR medium. a Undifferentiated IMR90 cells maintained in mTeSR were dissociated as single cells seeded on Matrigel from BD Biosciences (B-Matrigel, blue), from Corning (C-Matrigel, orange), from ThermoFisher (L-Matrigel, red) or from Trevigen (T-Matrigel, purple) at 20 × 103 cells/cm2. After 24, 48 and 72 h following cell seeding, IMR90 iPSC colonies were dissociated and counted using a cell hemocytometer and Trypan blue exclusion assay. b BMEC yield after 8 days of differentiation obtained by calculating the ratio of cell density at 8 days of differentiation versus the initial cell density seeded at day 0, ** denotes P < 0.01 in comparison to B-Matrigel. c Representative immunostained micrographs of PECAM-1, VE-cadherin, Glut-1 Claudin 5 and Occludin expression in purified BMEC monolayers 48 h post-purification. Note the formation of a monolayer as marked by defined tight junction complexes in one focal plane and the absence of junctional cellular overlap. Scale bar = 20 µm