Figure 4
Quantification of OT-I CD8+T cell interactions with the spinal cord microvasculature in vivo. A: Evaluation of the initial contact fraction (%) of OT-I CD8+ T cells with the post capillary venules (20–60 μm diameter) of the spinal cord microvasculature of mice with EAE. Each dot represents 1 venule. All values show the median with the interquartile range of n = 22 analyzed post-capillary venules from 3 mice for rat IgG2b condition, n = 18 analyzed post-capillary venules from 5 mice for anti- α4β7 condition, n = 18 analyzed post-capillary venules from 6 mice for anti- β7 condition and n = 23 analyzed post-capillary venules from 4 mice for anti-α4 condition. B: Shows the evaluation of capture and rolling fractions (%) of OT-I CD8+ T cells with the post capillary venules (20–60 μm diameter) of the spinal cord white matter microvasculature of mice afflicted with MOG35-55-induced EAE. N = 22 analyzed post-capillary venules from 3 mice for rat IgG2b condition, n = 18 analyzed post-capillary venules from 5 mice for anti- α4β7 condition, n = 18 analyzed post-capillary venules from 6 mice for anti-β7 condition and n = 23 analyzed post-capillary venules from 4 mice for anti-α4 condition. Statistical significance was determined by the Mann–Whitney U-Test.