Figure 14
From: Impedance-based cell monitoring: barrier properties and beyond
(A) Time course of normalized capacitance C of a sampling frequency of 40 kHz wounding of normal rat kidney (NRK) epithelial cells (wounding parameters: 4 V, 20 s, 40 kHz). The arrow indicates the time point of injury (2). (B) Confocal laser scanning microscopy (CLSM) images of the Live/Dead Assay show vital cells surrounding the active electrode surface (calcein acetoxymethylester stained in green) and dead cells (ethidium homodimer-1 stained in red) on the electrode itself. (B; 1-4) Documentation of the wound healing process by CLSM images, 1 = before wounding, 2 = after wounding, 3 = after partial wound healing, 4 = after complete wound healing. From [34] with permission.