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Table 2 Dose-dependent effect of cystatin C activity-blocking antibody

From: Measurement of cystatin C functional activity in the cerebrospinal fluid of amyotrophic lateral sclerosis and control subjects

Sample

CSF to antibody ratio (by volume)

Mean assay activity (RFU/min)

1

0:4

3737

2

4:0

754

3

4:1

2455

4

4:2

3944

5

4:3

3799

6

4:4

3603

  1. A CSF sample with the highest cystatin C concentration was pre-incubated with varying amounts of polyclonal cystatin C activity-blocking antibody (samples 2–6) and then tested in the papain inhibition assay. Sample 1 demonstrates the baseline papain assay activity in the absence of CSF-mediated papain inhibition and in the presence of the highest dose of anti-cystatin C antibody used in the experiment. Sample 2 demonstrates the maximal papain assay inhibition produced by the CSF without any anti-cystatin C antibody. Addition of the blocking antibody in samples 3–6 produces a dose-dependent elimination of CSF-mediated assay inhibition, with full elimination reached at a 4:2 ratio (where assay activity first returns to baseline). Cystatin C antibody was prepared at a 0.2 μg/μL concentration.
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Fluids and Barriers of the CNS

ISSN: 2045-8118