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Figure 1 | Fluids and Barriers of the CNS

Figure 1

From: Measurement of cystatin C functional activity in the cerebrospinal fluid of amyotrophic lateral sclerosis and control subjects

Figure 1

Depletion of cystatin C from CSF by immunoprecipitation. Lanes 1–4: Increasing amounts of anti-cystatin C polyclonal antibody (0–1.6 μg antibody) was added to CSF for immunoprecipitation as described in Methods. The resulting immunoprecipitate was analysed by western blot for cystatin C. Lane 1: 1.6 μg antibody (IP1); Lane 2: 0.8 μg antibody (IP2); Lane 3: 0.4 μg antibody; (IP3) Lane 4: 0 μg antibody (IP4). The remaining column flow-through from each sample was then used to repeat the cystatin C immunoprecipitation using 0.8 μg antibody to examine efficiency of cystatin C removal during the initial immunoprecipitation. Lane 5: Flow-through from IP1; Lane 6: Flow-through from IP2; Lane 7: Flow-through from IP3; Lane 8: Flow-through from IP4; Lane 9: Human cystatin C purified protein as a positive control from a separate gel.

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Fluids and Barriers of the CNS

ISSN: 2045-8118