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Figure 5 | Fluids and Barriers of the CNS

Figure 5

From: Na,K-ATPase alpha isoforms at the blood-cerebrospinal fluid-trigeminal nerve and blood-retina interfaces in the rat

Figure 5

Representative immunofluorescence images of Na,K-ATPase alpha-1, -2, and −3 expression at the rat retina. Na,K-ATPase isoforms labeled with fluorescein in the left hand column images, vWF visualized with Texas red in the second column, nuclei are DAPI-labeled, and the merged image for each horizontal panel is in the right hand column. Lower horizontal images are negative controls when no primary antibody was used. Unique Na,K-ATPase alpha-1 & -3 specific expression in the retinal cell layers is seen, similar to previous reports [23, 44]. The arrow indicates alpha-2 expression in the capillaries at the retinal ganglion cell layer. GC: ganglion cell layer; IN: inner nuclear layer; ON: outer nuclear layer are labeled for orientation on the alpha-3 DAPI image. Scale bar = 50 μm.

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Fluids and Barriers of the CNS

ISSN: 2045-8118