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Figure 4 | Fluids and Barriers of the CNS

Figure 4

From: Na,K-ATPase alpha isoforms at the blood-cerebrospinal fluid-trigeminal nerve and blood-retina interfaces in the rat

Figure 4

Representative immunofluorescence images of the blood-aqueous barrier at the rat ciliary body with Na,K-ATPase alpha-1, -2, and −3 isoform expression. Na,K-ATPase isoforms labeled with fluorescein in left hand column images, vWF visualized with Texas red in second column, nuclei are DAPI stained, and the merged image for each horizontal panel is in the right hand column. Lower horizontal images are negative controls with no primary antibody. Merged images show alpha-1 expressed on the PE, alpha-2 on the NPE, and alpha-2 expressed on ciliary body capillaries. The arrowhead points to the PE on the alpha-1 merged image, and the arrow points to the NPE on the alpha-2 merged image. Scale bar = 20 μm.

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Fluids and Barriers of the CNS

ISSN: 2045-8118