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Figure 3 | Fluids and Barriers of the CNS

Figure 3

From: Na,K-ATPase alpha isoforms at the blood-cerebrospinal fluid-trigeminal nerve and blood-retina interfaces in the rat

Figure 3

Representative immunofluorescence images of a choroid plexus from the 3rd ventricle, sequentially acquired at 1 μm steps in a z-stack to reveal the cellular location of Na,K-ATPase alpha-1 and −2 isoforms. The same Na,K-ATPase expression patterns were found in the choroid plexuses in the lateral ventricles (data not shown). Texas red is used to visualize alpha-1 and fluorescein for alpha-2, and the nuclei are DAPI stained. Negative controls (without primary antibody; data not shown) had no membrane or cytoplasmic staining. Scale bar = 10 μm.

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