Pathophysiology of communicating hydrocephalus in two novel animal models

  • Jie Li1,

    Affiliated with

    • Yimin Shen1,

      Affiliated with

      • Janet M Miller1Email author,

        Affiliated with

        • Michael R Egnor2,

          Affiliated with

          • Mark Wagshul2,

            Affiliated with

            • Curt Stewart3,

              Affiliated with

              • E Mark Haacke1,

                Affiliated with

                • Marion E Walker4,

                  Affiliated with

                  • Steven D Ham1 and

                    Affiliated with

                    • James P McAllister1

                      Affiliated with

                      Cerebrospinal Fluid Research20063(Suppl 1):S21

                      DOI: 10.1186/1743-8454-3-S1-S21

                      Published: 21 December 2006

                      Background

                      Communicating hydrocephalus (CH) occurs frequently but clinically-relevant animal models amenable to diagnostic imaging and cerebrospinal fluid shunting do not exist. This deficiency has inhibited our understanding of the pathophysiology of CH, which may be quite different from obstructive hydrocephalus. The purpose of this study was to perfect two novel models of CH and to characterize the histopathology that occurs in each.

                      Materials and methods

                      Two methods were employed using 25% kaolin or saline injected into the sub-arachnoid space (SAS). For injections into the basal cisterns (n = 7), after anterior exposure of the C1-clivus interval, a blunt 30–36 gauge needle was advanced into the SAS and 20–50 μl of kaolin was injected. For the cortical approach (n = 9), a craniotomy was created over both cerebral hemispheres, and a curved, blunt tip 25G needle was advanced gently into the SAS. After separating the partitions in the SAS, a total of 50–60 μl of kaolin was injected. Animals were sacrificed after chronic ventriculomegaly had developed, and the brains analyzed for cytoarchitectural changes, astrocytosis, microgliosis, neuron death, and axonal degeneration.

                      Results

                      All rats with kaolin injections appeared relatively normal, but preliminary motor learning testing showed transient neurological deficits. In rats with ventral brainstem injections, kaolin was observed grossly in the basal cisterns, but not into the cerebello-pontine angle, indicating that communicating – not obstructive – hydrocephalus had been induced. MRI studies with gadolinium injections into the lateral ventricle confirmed these findings. MRI also revealed that ventriculomegaly progressed steadily in 86% of these animals and in 2–14 days post-kaolin the mean Evan's ratio (0.43) increased significantly. In rats with cortical injections, kaolin covered approximately 80% of the hemispheres, and 89% developed mild protracted (4 months) ventriculomegaly (mean Evan's ratio 0.39) not significantly different from controls. In the periventricular white matter, the number of reactive astrocytes and microglia increased only after basal cistern injections. No degeneration of neuron cell bodies was noted in either injection group, but axonal degeneration was present in the periventricular white matter after basal cistern obstruction. These cytological changes correlated with the severity of ventriculomegaly.

                      Conclusion

                      These preliminary results suggest that CH can be induced with blockage of the cortical SAS and basal cisterns, that ventriculomegaly is much more protracted in the former model, and the time course of hydrocephalus correlates with the extent of cytological damage. Ongoing studies will help reveal why the time course of ventriculomegaly is so different in these two models.

                      Authors’ Affiliations

                      (1)
                      Wayne State University
                      (2)
                      Neurosurgery/HSC T12-080 SUNY, State University of New York
                      (3)
                      (4)
                      Department of Neurosurgery, Primary Children's Hospital

                      Copyright

                      © Li et al; licensee BioMed Central Ltd. 2006

                      This article is published under license to BioMed Central Ltd.

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