Figure 2
From: Choroid plexus epithelial monolayers – a cell culture model from porcine brain
A) Amplified cDNA of liver (control), fresh CP, CP 9 DIC, and 14 DIC in an ethidium bromide gel. TTR amplifies as 371 bp (top row), β-actin, used as internal standard amplifies at 703 bp (bottom row). For each sample, lanes 1 through 5 show amplification results for 15 to 35 cycles, with lanes corresponding to PCR products with 5 cycles of additional amplification (lane 1, 15; lanne 2,20, lane 3,25; lanne 4,30; lane 5, 35). B) Confocal images of choroid plexus epithelial cell monolayer 14 DIC. On the left, TTR is shown in green after incubation of the cells with anti-TTR and secondary antibody. Cell nuclei are shown in red (propidium iodide staining). No TTR staining was seen in control images (right side), when cells were incubated with secondary antibody only. C) TTR Western blot of liver (lanes 1 and 2), freshly isolated CP (lanes 3 and 4) and choroid plexus epithelial cells 14 DIC (lanes 5 and 6). Samples without primary antibody served as controls (lanes 1, 3 and 5).