Figure 4
From: The distribution of the anti-HIV drug, tenofovir (PMPA), into the brain, CSF and choroid plexuses
The CNS uptake of [3H]PMPA in the presence of unlabelled PMPA, antiretroviral drugs and probenecid at a perfusion time of 20 min. The cerebrum, cerebellum, pituitary and choroid plexuses values of [3H]PMPA were corrected for vascular space or extracellular space, as measured by D-[14C]mannitol. The CSF uptake of [3H]PMPA is uncorrected for D-[14C]mannitol, as in this tissue it represents a paracellular permeability marker molecule. The uptake of D-[14C]mannitol in the presence of any of the additional drugs was not significantly different to that achieved in their absence (control) and demonstrates that at these concentrations of unlabelled drugs the integrity of the barriers is maintained. The presence of these additional drugs also did not significantly affect the distribution of [3H]PMPA. Each time point represents mean ± S.E.M., n = 3–7.