Volume 2 Supplement 1

49th Annual Meeting of the Society for Research into Hydrocephalus and Spina Bifida

Open Access

In moderate communicating hydrocephalus of human fetuses, ependymal denudation is a common feature that may result in abnormal neurogenesis

  • María Dolores Domínguez1,
  • Antonio J Jiménez1Email author,
  • Patricia Páez1,
  • Bernardo Weil2,
  • Miguel Angel Arráez3,
  • Esteban M Rodríguez4 and
  • José-Manuel Pérez-Fígares1
Cerebrospinal Fluid Research20052(Suppl 1):S5

DOI: 10.1186/1743-8454-2-S1-S5

Published: 30 December 2005

Background

Recent investigations carried out in natural and experimental mutant mice have provided strong evidence that a primary alteration of the ependymal cell lineage triggers a moderate foetal hydrocephalus [1, 2]. In human cases of hydrocephalus, however, ependymal loss has been regarded as resulting from the ventricular dilatation due to the accumulation of cerebrospinal fluid [3].

Materials and methods

The present investigation was carried out in 16–40 week old human foetuses with a communicating hydrocephalus and displaying a moderate dilatation of the ventricular cavities (n = 8), and foetuses of similar ages with no neuropathological alterations (n = 15). Paraffin sections throughout the walls of the cerebral aqueduct and lateral ventricles were processed for lectin binding and immunocytochemistry using ependyma, astroglia, neuroblasts and macrophague markers.

Results

Large areas of ependymal denudation were found in the aqueduct and lateral ventricles of all foetuses developing a communicating hydrocephalus. At variance, no ependymal detachment was observed in non-hydrocephalic foetuses. In the youngest foetuses with hydrocephalus, denuded areas were not covered by astrocytes or other organized cell elements, leaving the neuropile directly exposed to the ventricular lumen. The area devoid of ependyma increased as the foetus developed. In the oldest foetuses studied, the denuded areas of the lateral ventricles were lined by a dense plexus of astrocytes. Under the denuded surface the presence of ependymal rosettes was observed. In the denuded areas of the lateral ventricles of hydrocephalic foetuses it was found (i) a loss of the germinal ependymal zone, (ii) disorganization of the subventricular zone and, (iii) abnormal migration of neuroblasts into the ventricular cavity.

Conclusion

The early loss of ependyma in human hydrocephalic foetuses would be associated to both, the hydrocephalic process and an abnormal migration of neuroblasts.

Declarations

Acknowledgements

Supported by grants from FIS, PI 030756 and Red CIEN C/0306, Instituto de Salud Carlos III, and Servicio Andaluz de Salud, Spain to JMP-F; and Fondecyt 1030256, Chile to EMR.

Authors’ Affiliations

(1)
Departamento de Biología Celular, Genética y Fisiología, Universidad de Málaga
(2)
Servicio de Anatomía Patológica Hospital Carlos Haya
(3)
Servicio de Neurocirugía Hospital Carlos Haya
(4)
Instituto de Histología y Patología, Universidad Austral de Chile

References

  1. Jiménez AJ, Tomé M, Páez P, Wagner C, Rodríguez S, Fernández-Llebrez P, Rodríguez EM, Pérez-Fígares JM: A programmed ependymal denudation occurring during the embryonic life precedes development of congenital hydrocephalus in the mutant mouse hyh. J Neuropathol Exp Neurol. 2001, 60: 1105-1119.PubMedGoogle Scholar
  2. Davy BE, Robinson ML: Congenital hydrocephalus in hy3 mice is caused by a frameshift mutation in Hydin, a large novel gene. Hum Mol Genet. 2003, 12: 1163-1170. 10.1093/hmg/ddg122.View ArticlePubMedGoogle Scholar
  3. Sarnat HB: Ependymal reactions to injury. A review. J Neuropathol Exp Neurol. 1995, 54: 1-15.View ArticlePubMedGoogle Scholar

Copyright

© The Author(s) 2005

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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