Table 1 Summary of the immunohistochemical characterization of CVOs in health and EAE
Molecule | Healthy1 | EAE2 |
|---|---|---|
Microvessels | ||
MECA-32 | + on all vessels | + on all vessels |
PECAM-1 | + on all vessels | + on all vessels |
ICAM-2 | + on some vessels | + on some vessels |
ICAM-1 | No staining | + on most vessels luminally and abluminally/perivascular |
VCAM-1 | No staining | + on few vessels luminally and abluminally or perivascular no luminal VCAM-1 detected in ME |
MAdCAM-1 | No staining | no staining |
E-selectin | no staining | no staining |
P-selectin | no staining | no staining |
Immune cells | ||
CD45 | + on few perivascular cells | + on many cells within the CVOs |
CD3 | no staining | + on few round cells ca. 4 cells/section in SFO ca. 10 cells/section in AP |
CD4 | no staining | + on few round cells |
CD8 | no staining | + on few round cells |
B220 | no staining | + on few round cells |
F4/80 | only rarely observed on single perivascular cells* | + on many exclusively perivascular cells |
Mac-1 (αM-integrin) | only rarely observed on single perivascular cells* | + on round and branched cells located perivascularly and throughout the CVOs |
LFA-1 (αL-integrin) | only rarely observed on single perivascular cells* | + on round and branched cells located perivascularly and throughout the CVOs |
α4-integrin | no staining | very faint staining on few round cells – not regularly detected |
MHC class I | only rarely observed on few perivascular cells§ | considerable staining throughout the CVOs; negative on endothelium |
MHC class II | only rarely observed on single perivascular cells* | + on many perivascular cells |